![]() Select the antiparallel sheet view in the pdb file. Locate the N- and C-termini in this image. Part - Exploring antiparallel B sheet structure The hydrogen bonding between antiparallel sheets, also visible in the pdb by selecting the antiparallel sheet view, is shown. Asp 117 The N-terminal amino acids are Val11. O chain number in the quaternary structure atom type (O is oxygen) atom number along the amino acid chain #916 Select the hydrogen bonds between three sheets View in the pdb file, then hover over atoms in the Jmol image showing hydrogen bonding to identify the N-terminal amino acids. Term Meaning [ASP) three-letter abbreviation for the amino acid 117 residue number in the protein sequence A. As an example, you might see the label (ASP]117:A.O #916. If you hover over the atoms in the Jmol image, identifying information will appear. Part B - Identifying N-termini in a B sheet The hydrogen bonding among three B strands is shown with standard CPK coloring of atoms, also visible in the pdb by selecting the hydrogen bonds between three sheets view. ![]() In the pdb, select to view the secondary structure, and determine the number of B strands found in GFP. strands that run parallel to each other to form a planar sheetlike surface that may be GFP has one continuous sheet creating the B-barrel. A B sheet consists of two or more twisted. It is one sheet that wraps around to form a cylinder, or barrel. The overall structure of GFP is called a B-barrel. Part A - Exploring B sheet structure Look at the model of GFP from the pdb. Other fluorescent proteins in this family glow red, yellow, or blue, so expression of multiple proteins can be studied simultaneously. You may have seen images of glowing mice. If the GFP gene is fused to another protein then cloned into an organism, cells that express the protein glow green. This protein has been "hijacked" by scientists to use as a tool for determining protein expression in various tissue types. In this item you will explore B sheet structure using green fluorescent protein (GFP), the protein that causes jellyfish to glow.
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